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Description
Glutathione reductase regenerates reduced GSH from its oxidized form (GSSG) using NADPH, predominantly sourced from the pentose phosphate pathway

Tang, Hu, Rui Kang, Jiao Liu, and Daolin Tang

As the immune system takes priority in survival, insufficient glutathione may express itself as fatigue since skeletal muscles cannot sustain normal aerobic metabolism
Click reaction mixture was prepared by sequential addition of 1 volume 10 mM azide-PEG3-biotin (Sigma, 762024), 2 volumes 50 mM CuSO 4 (Sigma, C8027), 2 volumes 50 mM TCEP (Sigma, 68957) and 1 volume 10 mM TBTA (Sigma, 678937)
