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Plasmonic characteristics of nanorod-based metallic nanostructures
Finally, MVEs fuse with lysosomes initiating receptor proteolysis and downregulation at the cell surface (Hanyaloglu and von Zastrow, 2008
doi: 10.1158/0008-5472.CAN-22-3260 83 EntezariMSadrkhanlooMRashidiMAsnafSETaheriazamAHashemiMet al
Briefly, cells were lysed (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5 U/L RNaseOUT, 1 X Roche Protease Inhibitor Cocktail), incubated with Anti-HuR (Molecular Probes, mp21277), sonicated to disrupt RNA, and bound to BSA/Salmon Sperm blocked Magnabeads (Invitrogen) for 16 h at 4 C
