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In many experimental animal models, MTX has been found to increase serum urea and creatinine levels (Reference Moodi, Hosseini and Abedini16,Reference Tousson, Zaki and Abu-Shaeir50,Reference Jafaripour, Naserzadeh and Alizamani51) , but there are also studies showing that MTX reduces serum urea concentration (Reference Budancamanak, Kanter and Demirel52Reference Severin, Campagno and Brandoni54)

For a quantitative insight, this finding was further confirmed by flow cytometry (flow cytometry plots: Fig

Epilepsy group Nineteen patients had epilepsy without fulfilling criteria of encephalitis

The FC between the two groups indicated is expressed as log 2 ratio