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The loaded biosensors were immersed in serial dilutions of the appropriate GATE16 construct at the noted concentrations at an orbital shake speed of 1,000 rpm at 30C for a 250-s association step, with a dissociation step in BLI buffer for 500 s between each concentration

Crosstalk between Metabolic Alterations and Altered Redox Balance in PTC-Derived Cell Lines

Qian Ren, Hebei Medical University, China Updates Copyright 2021 Freo, Brugnatelli, Turco and Zanette

Using an efficient method employing wheat germ agglutinin (WGA)-coupled magnetic beads for EV isolation, they identified three significantly elevated miRNAs miR-574-3p, miR-542-3p, and miR-200a-3p in MASLD patients compared to healthy controls
