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doi: 10.3233/JPD-161040

For co-IP, proteins from primary astrocytes were harvested by lysis in cell lysis buffer (150 mM NaCl, 0.5% NP-40, 10% glycerol, 2 mM mercaptoethanol, 0.2 mM phenylmethylsulfonyl fluoride, and 10 mM glycerophosphate) after experimental treatments

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Why does the water have to be injected down the side of the vial
