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A comparative study with the liver enzyme
Additionally, a key contribution of this review is its assessment of methodological limitations in the current literature
Even a single temperature excursion above 8Csuch as leaving the vial on a countertop for several hourscan denature the peptide structure, turning an effective compound into an inactive solution
Immunoprecipitated chromatin was eluted with the ChIP direct elution buffer (0.5% SDS, 5 mM EDTA, 10 mM Tris-HCl [pH 8], 300 mM NaCl) and incubated at 65 C for 4 h to reverse the crosslinks
