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Purification of the fusion protein is performed by immobilized metal affinity chromatography (IMAC) which has been comprehensively reviewed in the literature (Block et al., 2009, Charlton and Zachariou, 2007, Cheung et al., 2012, Gaberc-Porekar and Menart, 2001, Porath, 1992)
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doi: 10.1074/jbc.M109.048777

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