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Current models for studying microbial TMA production have numerous weaknesses including the cost and length of human studies, differences in TMA(O) metabolism in animal models and the risk of failing to replicate multi-enzyme/multi-strain pathways when using isolated bacterial strains

To summarize, there are a variety of affinity ligands that interact with protein or peptide tags for purification that include metal ions, small molecules, peptides, and proteins (Table 2)

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