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While insufficient inositol metabolism may lower TSH signal transduction effectiveness [7], selenium shortage has been associated with decreased selenoprotein activity, increased oxidative stress, and an increased risk of autoimmune thyroiditis [2,18,36,47]

Antioxid Redox Signal 15 , 551589, (2011)

Stimulation of neutrophils with PMA or bacteria leads to a rapid increase in ROS production, thereby triggering extensive DNA damage, which ultimately results in chromatin decondensation and NETosis [190]

The cells were incubated with blocking buffer (1% (w/v) BSA, 22.52 mg/ml glycine, 0.1% (v/v) tween-20) for 30 mins and washed with PBS three times