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Lastly, we did not observe any particular pattern of enzyme essentiality or the degree of activation for early or late regulated enzymes of the pathway (Fig

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The resulting mixture was combined with 80 l of 25 M N-acetylcysteine (internal standard), reduced using 20 l of 1.58 M N-ethylmorpholine and 8 l of 25 mM tris-(2-carboxyethy l) phosphine, and labeled with 8 l of 30 mM monobromobimane

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