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The supernatant was discarded, and the cell pellets were stored at 80C until further use

Moreover, dynamic PDU procedure-related anxiety might occasionally determine an incomplete pharmacologically induced erection, due to the increased sympathetic stimulation and adrenergic tone resulting from situational, non-constitutive, emotional disturbances deriving from the procedure, leading to an incomplete relaxation of cavernous smooth muscle and ensuing decreased blood inflow into the corpora cavernosa of the penis [43]

Zwingmann C, Chatauret N, Leibfritz D, Butterworth RF (2003) Selective increase of brain lactate synthesis in experimental acute liver failure: results of a [H-C] nuclear magnetic resonance study

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