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Covalent binding NAPQI is believed to exert its toxic effects by binding covalently to liver proteins leading to protein denaturation and necrosis of liver cells[6, 26, 27]

Comparative Benefits and Harms of Complementary and Alternative Medicine Therapies for Initial Treatment of Major Depressive Disorder: Systematic Review and Meta-Analysis

J Ethnopharmacol 257:112884 Attia GM, Elmansy RA, Elsaed WM (2019) Neuroprotective effect of nilotinib on pentylenetetrazol-induced epilepsy in adult rat hippocampus: involvement of oxidative stress, autophagy, inflammation, and apoptosis [J]

This manipulation maintains the chromophore maturation ability of the fluorescent protein but relaxes the structure relative to native YFP and its relatives, such that the site of chromophore maturation is more accessible to environmental influence (Kostyuk et al., 2019)