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The fold change in the target gene expression in the GSTM1 -positive versus the GSTM1 -null samples was calculated using the formula 2 CT following the general guidelines discussed by Schmittgen and Livak 33

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The cleavage of Snitrosyl group can occur spontaneously in the case of exposure to reducing agents (ascorbate, GSH), metal ions (Cu 2+ ), UV radiation, ROS, or nucleophiles [12] or can be catalyzed by denitrosylases that remove the nitrosyl group from Snitrosothiols

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