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Octanoylation appears to be essential for GH secretagogue activity, as des-acyl ghrelin is inactive for GH release (329)

Fluorometric assay of nitrite and nitrate in brain tissue after traumatic brain injury and cerebral ischemia
J Biol Chem 96:387395 [Google Scholar] Moncada S, Palmer RM, Higgs EA (1991) Nitric oxide: physiology, pathophysiology, and pharmacology
Many researchers run GLOW in six to eight week cycles with breaks in between, so temporary discontinuation is built into standard protocols
