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The procedure is based on the reduction of Ellmans reagent by SH groups to produce 5,5-dithiobis (2-nitrobenzoic acid) which has an intense yellow color that is measured spectrophoto- metrically at 412 nm using Thermo Scientific Genesis 10S spectropho- tometer

Many of the technical problems have been solved (see the reviews cited below), and mass spectrometric methods are suited to routine screening of large numbers of samples of biological fluids from neonates, as they permit a considerable degree of automation, both of the analytical steps and of the gathering and interpretation of data, although there appears to be a need for a standardized methodology