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In contrast, short-chain fatty acids at least up to octanoate can permeate the inner mitochondrial membrane in a non-esterified state, and they are activated to their CoA-derivatives in the mitochondrial matrix for use in energy-dependent mitochondrial processes

The analytical method involved the use of a surrogate matrix solution (sodium chloride, potassium chloride, dibasic sodium phosphate, monobasic sodium phosphate, and bovine serum albumin) to help reduce any matrix interference from the plasma samples