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Pulldown assay Wild-type (WT) and mutant GSTs were expressed in Rosetta (DE3) competent cells by 1 mM isopropyl -D-1-thiogalactopyranoside (IPTG) (Amnesco)

1 2 Annual Review of Biochemistry

doi:10.1056/NEJMoa1705835 Annane, Djillali et al

adoptive transfer of Tregs in DKD rat models restored the Th17/Treg balance and reduced albuminuria (24)
