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O 2 interacts with reduced FMN to generate and produced in Complex I, which is released into the mitochondrial matrix and converted to H 2 O 2 by manganese superoxide dismutase (MnSOD)

For analyses using Coomassie Blue staining, 10 pmol of either Scc2-Scc4, Scc2C or Scc4-Scc2N were incubated with F7 anti-HA antibody and protein A-conjugated magnetic beads at 4 C for 1 h in IP buffer

The DPPH scavenging activity assay determined the antioxidants' ability to neutralize DPPH radicals by donating electrons, resulting in a color shift measured with a spectrophotometer 65

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