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At that time, I was experiencing what I felt were signs and symptoms associated with low testosterone
Studies attempting to restore long-distance axonal connections typically aimed to create a permissive environment for axonal outgrowth, and/or augment the intrinsic capacity of axons to regenerate
Biofilms were washed once with 1 PBS and stained with a live/dead stain (Bacterial Viability Kit, Molecular Probes, Thermo-Fisher Scientific, USA), followed by incubation in the dark for 45 min before imaging
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