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Unreacted sites were capped with ethanolamine (1.0 M ethanolamine in 10 mM PBS, pH 8.1, 7 min)
Protein-inhibitor complex was prepared by incubating 50 M of the 3CL-GP-6xHis with 500 M of inhibitor in a buffer comprised of 50 mM Tris-HCl (pH 7.5), 1 mM EDTA for 1 h at 4 C before immobilization onto beads
[DOI] [PMC free article] [PubMed] [Google Scholar] 95.Miricescu D, Totan A, Calenic B, Mocanu B, Didilescu A, Mohora M, et al
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