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RT-PCR An RNeasy kit (Qiagen, 74104) was used to extract total RNA from mice hippocampal tissue, and then the Transcriptor High Fidelity cDNA synthesis kit (Roche, 5081963001) was used to reverse transcribe the RNA into cDNA

Stomach acid is necessary to release vitamin B12 from food, and intrinsic factor is required for its absorption

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The omega-3 fatty acids commonly obtained from fish, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), inhibit signaling of a pro-inflammatory molecule called nuclear factor-kappa B (NF-B) and reduce levels of other chemicals that trigger inflammation, such as tumor necrosis factor-alpha (TNF-) and interleukin-6