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Rats were acclimatized to a controlled environment of 22 1 C temperature and a 12-h light/dark cycle, with free access to food and water, for 2 weeks prior to the experiments

10 mg vial + 3 mL bacteriostatic water 10 mg 3 mL = 3.33 mg/mL At this concentration the same 0.25 mg starting dose becomes 0.25 3.33 = 0.075 mL 7.5 units , and the 2.4 mg maintenance dose becomes 2.4 3.33 = 0.72 mL = 72 units

Bioavailability of Intranasal Drug Delivery System

Pharmacokinetics of Caffeine The way the body reacts to a drug is explained by pharmacokinetics