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In particular, germline mutations convey pro-tumorigenic features and often affect the high-risk susceptibility genes CDKN2A and, less commonly, CDK4 , associated with familial melanoma, where the phenotype of CDKN2A or CDK4 -mutated families is indistinguishable ( The CDKN2A gene is the major high-penetrance familial melanoma predisposition gene, with germline mutations identified in 20%-40% of melanoma families ( CDKN2A mutations have been reported associated to MPMs in Italian patients, being more frequent in MPM cases with a positive family history ( The tumor suppressor CDKN2A is located at the 9p21 locus and encodes 2 different proteins, p16INK4A (p16) and p14ARF (p14), which promote the cell cycle arrest in G1 phase by inhibiting RB protein phosphorylation through CDK4 and act through the p53 pathway inducing cell cycle arrest or favoring apoptosis, respectively ( CDKN2A produce an imbalance between functional p16 and Cyclin D1, causing abnormal cell growth

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GHRP-6 was administered in doses from 10(-8) M to 10(-5)M as well as 10(-9)M in the pituitary cells of the cows