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Briefly, the detergent, DTT and other low-molecular-weight components were removed using 200 l UA buffer by repeated ultrafiltration facilitated by centrifugation

In the future, the integration of metabolomic readouts, single-cell and spatial transcriptomic localization, and metabolic modeling analysis is expected to establish a research framework of metabolic stratification-target selection-efficacy prediction, thereby reducing the risk of failed extrapolation across different diseases or populations (138)

[PubMed] [Google Scholar] Ruscoe JE, Rosario LA, Wang T, Gate L, Arifoglu P, Wolf CR, Henderson CJ, Ronai Z and Tew KD

Trentacoste et al., 2001