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For HTS in a 1536-well format, HEK293T cells were bulk-transfected using JetPRIME (Polyplus, New York) following the manufacturers recommendations

10.1038/nsmb.2754 [DOI] [PMC free article] [PubMed] [Google Scholar] Perrem K, Colgin LM, Neumann AA, Yeager TR, Reddel RR (2001) Coexistence of alternative lengthening of telomeres and telomerase in hTERT-transfected GM847 cells

Environmental factors can slow down the conversion of methylfolate to folinic acidand vice versa

Glutathione is also involved in restoring damaged cell components, mainly proteins and lipids of cell membranes and DNA
