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Participants on the 5mg dose lost an average of 16.0% of their body weight

Cells were washed with PBS to remove excessive NEM before lysing in triton lysis buffer (50 mM TrisHCl, pH 7.5, 1 mM EDTA, 1% (v/v) Triton X-100, 150 mM NaCl) supplemented with protease inhibitor cocktail (EMD Millipore, 539134)

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In relation to the values for the saline treated mice, naloxone reacted immediately, while BPC 157 required 30 minutes' period
