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Relative number of living cells is defined as the following equation: relative number of living cells = counted living cell number (sample)/counted living cell number (control), where the control is cells cryopreserved by a commercially available cryoprotectant, CultureSure freezing medium (CS-FM, Fujifilm Wako Pure Chemical Corporation)

With this approach we routinely quantify ~7000 phosphopeptides in just 15 min of LC-MS/MS analysis time with the fast 28 Hz higher-energy collisional dissociation (HCD) 10 scanning method on a Q Exactive HF-X mass spectrometer 8 with overall MS/MS identification rates of more than 50%

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