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In vitro cytotoxity tests proved that the nanogel was more toxic than TAC-THQ suspension gel on HaCaT cells and exhibited higher antioxidant activity comparable to free thymoquinone and skin permeation level
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When glutathione is involved in antioxidant reactions, it becomes the oxidized form, GSSG (glutathione disulfide)
This phenomenon is reminiscent of autoprotection observed in experimental animals, where initial exposure to the sub-toxic doses of acetaminophen protects rodents from subsequent lethal doses of the drug [59,60]
