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After three basal measurements, cells were permeabilized by injection of saponin (1 g/mL, Sigma S4521), together with 1 mM ADP (Sigma 01905) and the following respiratory complex substrates: CI, pyruvate/malate (5 mM/2.5 mM)

This deficiency can lead to a wide array of severe health problems (Figure 2), including megaloblastic anemia and irreversible neurological damage (13)

Therefore, whether different subtypes of DupA can protect the patients with duodenal ulcer from early gastric cancer warrants further exploration

One theory regarding the essential function of Sec at the active site of GPxs is that the presence of Sec instead of a Cys at the active site may enhance the rate of reaction with hydrogen peroxide because Sec is deprotonated at physiological pH