glutathione standard redox potential (E h ) of thiol redox couples during normal phenotypic Redox Reactions: Review – BIOC*2580:

The alcohol permeabilizes bacterial membranes, disrupting the proton motive force and interfering with energy-dependent cellular processes

Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template

this is the regulatory ceiling in both the US and the EU

Schwarz et al ., 2016)