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After washing 3 times with 0.1 M PBS, 10 min each, the cells were incubated with corresponding secondary antibodies in blocking solution in the following composition: conjugated Alexa 647 donkey-anti-chicken anti IgG (1:500, 703605-155, Jackson), Alexa 488 donkey anti-guinea-pig IgG (1:500, 706545-148, Jackson), Alexa 488 donkey-anti-rabbit IgG (1:200, 711547-003, Jackson) for 2 h at room temperature with gentle rocking

Two different models were compared

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After reduction, it is assimilated into bio-organic compounds, with cysteine being the first product (Figure 1: GSH biosynthesis)