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In brief, chemical standard libraries of 149 metabolites in seven pooled libraries were prepared and serially diluted in high-performance liquid chromatography (HPLC)-grade water from in a dilution series from 5 mM to 1 M to generate external standard pools, which are used for calibration of isotopically labelled internal standards and to quantify concentrations of metabolites for which internal standards were not available

The transcellular lipophilic pathway enables small, lipophilic molecules to diffuse across ECs, but limits the range of molecules that can be delivered [21]

To exclude any effects associated with the procedure used for generation of a new monoclonal sub-clone, wild-type HepG2 cells were treated with varying concentrations of NFT or DEM for 24 h, fixed, and subjected to immunocytochemistry to visualize Nrf2 localization (Fig

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