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Finally, an abnormal protein is cleaved into short peptide fragments, and the polyubiquitin chain is released and is trimmed into monomeric ubiquitin by deubiquitinating enzymes (DUBs) (Mevissen and Komander, 2017)

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Mixtures of calf thymus double-stranded (ds) DNA (Sigma Aldrich, Australia) at 150 ng/L with varying pyocyanin or phenazine (0, 25, 50 and 100 M) concentrations in Milli-Q water were incubated at room temperature (25 1C) for 15 min

Slowly inject the bacteriostatic water into the epithalon vial, allowing it to trickle down the side and gradually dissolving the epithalon powder