glutathione beads 4b Agarose Resin tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non Denaturing elution from glutathione agarose.
Description
The impact of autophagy on cuproptosis is not unidirectional but exhibits a dual regulatory role, capable of either promoting or inhibiting the process
Briefly, the supernatant is loaded onto the column in 6 M guanidine-HCl, pH 8, the column is first washed with 10 volumes of 6 M guanidine-HCl, pH 8, then washed with 10 volumes of 6 M guanidine-HCl pH 6, and finally the polypeptide is eluted with 6 M guanidine-HCl,pH5
doi: 10.1038/nchembio.2079 50 WangLLiuYDuTYangHLeiLGuoMet al
E.PhipsonB.WuD.HuY.LawC
