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Thermal cycling consisted of initial activation (95C for 7 min) followed by 35 cycles of denaturation (95C, 30 s), annealing (56C, 30 s), and extension (72C, 30 s) and a final extention at 72C for 7 min

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This includes evaluating potential adverse effects of high-dose antioxidant supplements and understanding their interactions with other medications or health conditions

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