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These effects on the TJ proteins by inflammatory cytokines is in part mediated by myosin light-chain phosphorylation through myosin light-chain kinase (MLCK)
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This disruption enhances oxidative stress, a precursor to chronic conditions like cardiovascular diseases ( Glutathione Peroxidase (GPX) and Catalase (CAT): Prolonged Zn supplementation led to reduced GPX and CAT activities in our study, amplifying hydrogen peroxide accumulation

Initiating NAC treatment within 8 h of overdosing minimizes the risk of hepatocellular damage as the effectiveness of NAC treatment is negatively correlated with the time post-overdose [28]
