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Furthermore, it will incorporate insights into metabolic reprogramming to provide a detailed analysis of the mechanisms driving SA-AKI and explore potential targeted therapeutic strategies, providing solid theoretical framework for the development of targeted therapies for SA-AKI

[ [PMID: 23420555]

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LCMS/MS analysis of phosphorylated Ycf1 To measure quantitative phosphorylation of purified Ycf1, 10 g of Ycf1 were Trypsin/LysC (Promega, Madison WI) digested with an S-Trap column (ProtiFi, Farmingdale NY) using the manufacturers suggested protocol following reduction with DTT and alkylation with IAA with ProteaseMax (0.1% Promega) added to the digestions
