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Three or more acyl-carnitines were abnormal a second time in 20 (48%) of the 42 patients, resulting in a prevalence of 17% of ASD children who manifested consistent acyl-carnitine panel abnormalities
RNA from microdissection samples were extracted in 300 L TRI-Reagent, precipitated in 500 L isopropanol overnight at 20 C, solubilized in 20 L of RNase-free water and quality checked using Total RNA 6000 Pico chips and the 2100 Bioanalyzer
Science 2001
