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FuS-PWangJFXueWJLiuHMLiuBRZengYLet al

Both urea and guanidinium chloride are powerful denaturants and would unfold the protein, if the protein binds to the resin in presence of these denaturants, it can be said with certainty that folded protein hides the his-tag and prevents its binding to the IMAC resin

Staying alive overdosed: how does Helicobacter pylori control urease activity

109113,259,292,336343 In addition, prolonged hypoxia causes renal endothelium to produce pro-inflammatory adhesion molecules, cytokines, and chemokines that activate macrophages already resident within the kidney and also recruit additional inflammatory cells from the circulation