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Elevated blood sugar and insulin blunt GH release
Diamide caused the formation of the TrxR1-hTrx1 complex as well as hTrx1 dimer (lanes 2,3,5 in Figure 4A, and lane 3 in Figure 4B), but not TrxR1 dimer (lane 4 in Figure 4A, lane 5 in Figure 4B)
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Upon elution, the mixture is resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed by Coomassie staining, silver staining, or Western blot
