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Free glutathione, GSH, is read first after 15 minutes, followed by addition of a reaction mixture that converts all the oxidized glutathione, GSSG, into free GSH, which then reacts with the excess ThioStar to yield the signal related to Total GSH content
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d , Viability of HEK293T cells after treatment with increasing amounts of TMEFF1-C peptide measured by LDH assay (n = 3 biological replicates)
