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After completion of the isoelectric focusing, the strip was equilibrated in the equilibration solution as described by Barraclough [37] and placed on a polyacrylamide gel for SDS-PAGE with a Protean II unit (Bio-Rad, USA) at a constant voltage (300 V)
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9 , 192200 (2013) Roozenbeek, B., Maas, A
Tumor Necrosis Factor-Alpha Is Required in the Protective Immune Response against Mycobacterium tuberculosis in Mice
Keep detailed records of injection times, dosages, and any observed reactions to maintain compliance and ensure accurate data collection
