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Membranes were blocked in 3% fat-free milk for 2 h at room temperature, then incubated overnight at 4 C in primary antibodies (Ku70 (1:1000) Cell Signaling Technologies D10A7, Actin (1:5000) Novus Biologicals nb600-535, Pol (1:1000) Abcam ab26343) diluted in 3% BSA
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[23] This deficiency contributes to alcohol intolerance by impairing the breakdown of acetaldehyde, a toxic intermediate produced during alcohol metabolism
MitoQ is one of the most popular mitochondria-targeted antioxidants
