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4e, microbial DNA extraction from fecal content was done using ZymoBIOMICS 96 Magbead DNA Kit automated on a Tecan Fluent 480 liquid handling system

Most protocols recommend 2-3 hours after eating, with sleep following within 30-60 minutes

In 65.1% of the samples, the declared active pharmaceutical ingredients (APIs) were present, whereas in 34.9%, they were not