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doi: 10.1167/iovs.13-12979 30 CastroVSkowronskaMLombardiJHeJSethNVelichkovskaMet al

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However, it should be noted that this concentration may not take into account protein-bound copper, and indeed, later experiments using a fluorescent probe along with ICP-MS found that following depolarization the concentration of free copper released from synaptosomes was likely to be closer to 23 M (Hopt et al., 2003)