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Before both assays were run, samples were deproteinized with the ReadiUse TCA Deproteinization Sample Preparation Kit (Biomol, ABD-19501)

doi: 10.1016/j.niox.2006.03.00

GenBank AA305856 EST176857 Colon carcinoma (Caco-2) cell line II Homo sapiens cDNA 5' end, mRNA sequence

Inhibition of ASCT2 significantly reduces glutamine uptake, cell proliferation, tumor growth, and metastasis by lowering E2F pathway proteins
