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For the activation assay, freshly isolated CD4 + CD25 + regulatory T cells were either maintained in a culture medium with 5 ng/ml -1 IL-2 or activated with 5 ng/ml -1 IL-2 and anti-mouse CD3/CD28 beads according to the manufacturers instructions (Gibco, Thermo Fisher Scientific)
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Chem Res Toxicol 24, 3034
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