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After incubation for 30 min, 50 l of the mixture were transferred to the wells of a black F16 Fluoronunc module (ThermoFisher Scientific, Nunc) containing 50 l of 10 mM fluorogenic thrombin substrate (Boc-Asp(OBzl)-Pro-Arg-AMC) and thrombin catalyzed substrate hydrolysis was monitored at ex of 360 nm and em of 460 nm using a Synergy 2 microplate reader (BioTek, Bad Friedrichshall, Germany)

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10.3 Manufacturing From a manufacturing perspective, several OSC subclasses are technically feasible for large-scale production

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