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Small interfering RNA (siRNA) targeting GPx4 (Applied Biosystems Life Technologies, s6112), NCOA4 (Applied Biosystems Life Technologies, s15566, s15567), NCOA4 (for in vivo experiment) (Applied Bio systems Life Technologies,s77518), ATG5 (Applied Biosystems Life Technologies, s18159, s18160) and negative control siRNAs (Applied Biosystems Life Technologies, AM4635, AM 4641) were purchased

Take with Vitamin C for improved absorption

Deficiency may result in macrocytic anemia and neurologic dysfunction

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