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Description
A., Hill, S

PMID: 31415071
In brief, the total volume of 3.0 mL reaction solution comprising 1.5 mL of 50 mM of phosphate buffer (pH 7.0), 0.2 mL enzyme extract, 1 mL of 1 M H 2 O 2 , and 0.3 mL distilled water was used to test the CAT activity by calculating the reduction in absorbance measured at 240 nm within 3 min

As mentioned before, insulin distributes along the trigeminal nerve and reaches the CNS after intranasal application in rats [14]